What is the difference between a slant and a deep

They tell me that it is one of the followingthree:. I can do gram staining to rule out e. Two from each of the followingCategories:. So far I am having a hard time finding the results of thesetests for B. Megaterium and B. How doI differentiate these two!? Please help! You have 0 free answers left. Get unlimited access to 3. Already have an account? Log in. Broth: Large number of bacteria that is easy to transport Agar Slants : Solid growth surface: easier to store and transport than plates.

Agar Deep : grow bacteria that requires less O2. Give three examples of growth patterns on agar slants draw or describe. What is the difference between agar slant and agar deep? Broth: Large number of bacteria that is easy to transport Agar Slants: Solid growth surface: easier to store and transport than plates.

Agar Deep: grow bacteria that requires less O2. Describe how you inoculated an agar slant using another agar slant that has bacteria on it. Which two tools are commonly used in transferring bacteria? Inoculating loops and needles made of metal wire can be used to transfer bacteria from one medium to another, such as from the surface of an agar plate to a broth. Metal tools may be sterilized by heating them in the flame of a Bunsen burner. What are some advantages of growing bacteria on agar plates?

Advantages: can accomodate up to 12 strains, low media usage especially if you use antibiotics, handling flexibility, minimal contamination. Disadvantages: streaking to isolate single colonies is very difficult due to small streaking area, culture media easily dry up. Why do you use a straight inoculating needle to inoculate an agar deep tube?

Prevents bacteria on the neck from entering or spreading through the neck of the culture. What is a straight needle used for? What is broth in microbiology? A clear liquid made from meat, vegetables or bones boiled in water and used to prepare other foods. A liquid medium—e. What does it mean to culture bacteria? Culture media is of fundamental importance for most microbiological tests: to obtain pure cultures, to grow and count microbial cells, and to cultivate and select microorganisms.

A microbiological culture medium is a substance that encourages the growth, support, and survival of microorganisms. What is the best way to dissolve agar in a medium as you are preparing it? It is solid at room temperature, but liquid at temperatures that are tolerated by bacteria.

Why is agar a useful solidifying agent in microbiology? Add several drops of HCl and recheck the pH. To dissolve them, you need to heat the solution first.

The only difference between broth and agar media is that broths do not contain an agar component. We use broth tubes primarily for specific assays, or rarely for bacteria that will not form colonies on a solid surface.

Unlike preparation of agar plates, tubes are prepared with media already in the incubation vessel. Bacterial liquid cultures should be shaken always but not too vigorously , in order to ensure aeration and oxygen and nutrient availability as well as to avoid bacterial settlement on the flask bottom which would result in cell death from the lack of nutrient availability.

Nutrient Agar is used for the cultivation of bacteria and for the enumeration of organisms in water, sewage, feces and other materials. Early in the 20th century, the American Public Health Associa- tion published the formula for a general purpose medium for the growth of a wide variety of nonfastidious microorganisms. Begin typing your search term above and press enter to search.

Press ESC to cancel. Skip to content Home Physics What is the primary use of the following agar slant agar deep broth? Ben Davis November 12, What is the primary use of the following agar slant agar deep broth?

What are agar deep used for? What is the difference between agar slants and deep Agar? What would you use to inoculate an agar deep? What tool is inoculate agar deep? Why is it important to use a loop rather than a needle to inoculate an agar plate? What are slant cultures used for? How do you make nutrient agar slants?